Active Demethylation of Paternal Genome in Mammalian Zygotes
نویسندگان
چکیده
منابع مشابه
Active demethylation of the paternal genome in the mouse zygote
DNA methylation is essential for the control of a number of biological mechanisms in mammals [1]. Mammalian development is accompanied by two major waves of genome-wide demethylation and remethylation: one during germ-cell development and the other after fertilisation [2] [3] [4] [5] [6] [7]. Most previous studies have suggested that the genome-wide demethylation observed after fertilisation oc...
متن کاملTet3 and DNA replication mediate demethylation of both the maternal and paternal genomes in mouse zygotes.
With the exception of imprinted genes and certain repeats, DNA methylation is globally erased during preimplantation development. Recent studies have suggested that Tet3-mediated oxidation of 5-methylcytosine (5mC) and DNA replication-dependent dilution both contribute to global paternal DNA demethylation, but demethylation of the maternal genome occurs via replication. Here we present genome-s...
متن کاملGenome-wide and gene specific paternal demethylation in androgenetic embryos.
In the present study, a genome-wide measure of demethylation employing the 5 methylcytidine antibody as well as a gene specific approach by bisulphite sequencing of a house keeping and imprinted genes was investigated to confirm if the active paternal demethylation was occurred correctly in mouse haploid, diploid androgenetic and triploid polyspermic embryos. The results indicated that the acti...
متن کاملGenome-wide Bisulfite Sequencing in Zygotes Identifies Demethylation Targets and Maps the Contribution of TET3 Oxidation
Fertilization triggers global erasure of paternal 5-methylcytosine as part of epigenetic reprogramming during the transition from gametic specialization to totipotency. This involves oxidation by TET3, but our understanding of its targets and the wider context of demethylation is limited to a small fraction of the genome. We employed an optimized bisulfite strategy to generate genome-wide methy...
متن کاملGSE Is a Maternal Factor Involved in Active DNA Demethylation in Zygotes
After fertilization, the sperm and oocyte genomes undergo extensive epigenetic reprogramming to form a totipotent zygote. The dynamic epigenetic changes during early embryo development primarily involve DNA methylation and demethylation. We have previously identified Gse (gonad-specific expression gene) to be expressed specifically in germ cells and early embryos. Its encoded protein GSE is pre...
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ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 2009
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.20234